ATP5A1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- extracellular vesicular exosome [ISO]
- membrane [ISO]
- mitochondrial inner membrane [IDA, ISO]
- mitochondrial proton-transporting ATP synthase complex [ISO]
- mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) [ISO]
- mitochondrion [IDA, ISO]
- myelin sheath [IDA]
- plasma membrane [ISO]
- proton-transporting ATP synthase complex, catalytic core F(1) [ISO]
PNPT1
Gene Ontology Biological Process
- RNA catabolic process [ISO]
- RNA import into mitochondrion [ISO]
- RNA phosphodiester bond hydrolysis, exonucleolytic [ISO]
- RNA polyadenylation [ISO]
- cellular response to interferon-beta [ISO]
- cellular response to oxidative stress [ISO]
- mRNA catabolic process [ISO]
- mitochondrial RNA 3'-end processing [ISO]
- mitochondrial RNA 5'-end processing [ISO]
- mitochondrial RNA catabolic process [ISO]
- mitochondrial mRNA catabolic process [IMP, ISO]
- mitochondrial mRNA polyadenylation [ISO]
- mitochondrion morphogenesis [IMP]
- mitotic cell cycle arrest [ISO]
- negative regulation of growth [ISO]
- nuclear polyadenylation-dependent mRNA catabolic process [ISO]
- positive regulation of mRNA catabolic process [ISO]
- positive regulation of miRNA catabolic process [ISO]
- positive regulation of mitochondrial RNA catabolic process [ISO]
- protein homooligomerization [ISO]
- protein homotrimerization [ISO]
- rRNA import into mitochondrion [ISO]
- regulation of cellular respiration [IMP]
- regulation of cellular senescence [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue.
While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically nonexistent. Here, we demonstrate the application of crosslinking mass spectrometry to identify protein structural features and interactions in tissue samples, providing systems ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence protein interaction identified through a combination of MS3 search results (Comet) from cross-linked peptide pairs enriched from two samples each. The results (2663 XL pairs) were filtered to a maximum evalue of 0.2 resulting in an estimated FDR at the PSM level of 0.007642.
Curated By
- BioGRID