DNAJA3
Gene Ontology Biological Process
- T cell differentiation in thymus [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- activation-induced cell death of T cells [IMP]
- apoptotic process [IMP]
- cell aging [IDA]
- embryo development [IMP]
- mitochondrial DNA replication [IMP]
- mitochondrion organization [IMP]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISA, ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of interferon-gamma-mediated signaling pathway [ISO]
- negative regulation of programmed cell death [IMP]
- negative regulation of protein kinase activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- neuromuscular junction development [IMP, ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of apoptotic process [ISO]
- positive regulation of protein ubiquitination [ISO]
- protein folding [ISO]
- protein refolding [IBA]
- protein stabilization [ISO]
- regulation of apoptotic process [IBA]
- regulation of catalytic activity [IDA]
- response to interferon-gamma [ISO]
- skeletal muscle acetylcholine-gated channel clustering [IMP]
- small GTPase mediated signal transduction [IDA]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- NF-kappaB binding [ISO]
- chaperone binding [IBA]
- interferon-gamma receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor tyrosine kinase binding [IPI]
- small GTPase regulator activity [IDA]
- transcription factor binding [ISO]
- unfolded protein binding [IPI]
- Hsp70 protein binding [ISO]
- NF-kappaB binding [ISO]
- chaperone binding [IBA]
- interferon-gamma receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor tyrosine kinase binding [IPI]
- small GTPase regulator activity [IDA]
- transcription factor binding [ISO]
- unfolded protein binding [IPI]
Gene Ontology Cellular Component
- I-kappaB/NF-kappaB complex [ISO]
- IkappaB kinase complex [ISO]
- actin filament [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- extrinsic component of plasma membrane [IDA]
- intracellular membrane-bounded organelle [ISO]
- mitochondrial matrix [ISO]
- mitochondrial nucleoid [ISO]
- mitochondrion [IDA, IMP, ISO]
- neuromuscular junction [IDA]
- nucleus [IDA, ISO]
- postsynaptic membrane [IDA]
DNAJA3
Gene Ontology Biological Process
- T cell differentiation in thymus [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- activation-induced cell death of T cells [IMP]
- apoptotic process [IMP]
- cell aging [IDA]
- embryo development [IMP]
- mitochondrial DNA replication [IMP]
- mitochondrion organization [IMP]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISA, ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of interferon-gamma-mediated signaling pathway [ISO]
- negative regulation of programmed cell death [IMP]
- negative regulation of protein kinase activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- neuromuscular junction development [IMP, ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of apoptotic process [ISO]
- positive regulation of protein ubiquitination [ISO]
- protein folding [ISO]
- protein refolding [IBA]
- protein stabilization [ISO]
- regulation of apoptotic process [IBA]
- regulation of catalytic activity [IDA]
- response to interferon-gamma [ISO]
- skeletal muscle acetylcholine-gated channel clustering [IMP]
- small GTPase mediated signal transduction [IDA]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- NF-kappaB binding [ISO]
- chaperone binding [IBA]
- interferon-gamma receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor tyrosine kinase binding [IPI]
- small GTPase regulator activity [IDA]
- transcription factor binding [ISO]
- unfolded protein binding [IPI]
- Hsp70 protein binding [ISO]
- NF-kappaB binding [ISO]
- chaperone binding [IBA]
- interferon-gamma receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor tyrosine kinase binding [IPI]
- small GTPase regulator activity [IDA]
- transcription factor binding [ISO]
- unfolded protein binding [IPI]
Gene Ontology Cellular Component
- I-kappaB/NF-kappaB complex [ISO]
- IkappaB kinase complex [ISO]
- actin filament [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- extrinsic component of plasma membrane [IDA]
- intracellular membrane-bounded organelle [ISO]
- mitochondrial matrix [ISO]
- mitochondrial nucleoid [ISO]
- mitochondrion [IDA, IMP, ISO]
- neuromuscular junction [IDA]
- nucleus [IDA, ISO]
- postsynaptic membrane [IDA]
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes.
Mitochondria exert an immense amount of cytophysiological functions, but the structural basis of most of these processes is still poorly understood. Here we use cross-linking mass spectrometry to probe the organization of proteins in native mouse heart mitochondria. Our approach provides the largest survey of mitochondrial protein interactions reported so far. In total, we identify 3,322 unique residue-to-residue contacts involving ... [more]
Throughput
- High Throughput
Additional Notes
- Cross-linking of proteins from native mouse heart mitochondria was carried out using the lysine-reactive DSSO.
- High confidence interactions had an FDR =< 0.02 (2% false discovery rate).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DNAJA3 DNAJA3 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3735131 | |
| DNAJA3 DNAJA3 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | - |
Curated By
- BioGRID