CYCS
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [IMP]
- apoptotic DNA fragmentation [ISO]
- apoptotic process [IDA]
- hydrogen peroxide metabolic process [IDA]
- mitochondrial electron transport, cytochrome c to oxygen [IBA]
- mitochondrial electron transport, ubiquinol to cytochrome c [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SLC25A4
Gene Ontology Biological Process
- apoptotic mitochondrial changes [IGI]
- ion transmembrane transport [ISO]
- negative regulation of cardiac muscle cell apoptotic process [ISO]
- negative regulation of mitochondrial membrane permeability involved in apoptotic process [ISO]
- negative regulation of necroptotic process [ISO]
- nucleotide transmembrane transport [ISO]
- positive regulation of cell growth involved in cardiac muscle cell development [ISO]
- positive regulation of oxidative phosphorylation uncoupler activity [ISO]
- purine nucleoside transmembrane transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes.
Mitochondria exert an immense amount of cytophysiological functions, but the structural basis of most of these processes is still poorly understood. Here we use cross-linking mass spectrometry to probe the organization of proteins in native mouse heart mitochondria. Our approach provides the largest survey of mitochondrial protein interactions reported so far. In total, we identify 3,322 unique residue-to-residue contacts involving ... [more]
Throughput
- High Throughput
Additional Notes
- Cross-linking of proteins from native mouse heart mitochondria was carried out using the lysine-reactive DSSO.
- High confidence interactions had an FDR =< 0.02 (2% false discovery rate).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLC25A4 CYCS | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.844 | BioGRID | 2668337 | |
| SLC25A4 CYCS | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3735296 |
Curated By
- BioGRID