ETFB
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ATP5B
Gene Ontology Biological Process
- ADP biosynthetic process [ISO]
- ATP biosynthetic process [ISO]
- ATP catabolic process [ISO]
- ATP metabolic process [ISO]
- angiogenesis [ISO]
- hydrogen ion transmembrane transport [ISO]
- lipid metabolic process [IMP]
- negative regulation of cell adhesion involved in substrate-bound cell migration [IMP]
- osteoblast differentiation [ISO]
- proton transport [ISO]
- receptor-mediated endocytosis [ISO]
- regulation of intracellular pH [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell surface [ISO]
- extracellular vesicular exosome [ISO]
- membrane [ISO]
- mitochondrial inner membrane [IDA, ISO]
- mitochondrial membrane [ISO]
- mitochondrial nucleoid [ISO]
- mitochondrial proton-transporting ATP synthase complex [ISO]
- mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) [ISO]
- mitochondrion [IDA, ISO]
- myelin sheath [IDA]
- nucleus [ISO]
- plasma membrane [ISO]
- proton-transporting ATP synthase complex, catalytic core F(1) [ISO]
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes.
Mitochondria exert an immense amount of cytophysiological functions, but the structural basis of most of these processes is still poorly understood. Here we use cross-linking mass spectrometry to probe the organization of proteins in native mouse heart mitochondria. Our approach provides the largest survey of mitochondrial protein interactions reported so far. In total, we identify 3,322 unique residue-to-residue contacts involving ... [more]
Throughput
- High Throughput
Additional Notes
- Cross-linking of proteins from native mouse heart mitochondria was carried out using the lysine-reactive DSSO.
- High confidence interactions had an FDR =< 0.02 (2% false discovery rate).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ETFB ATP5B | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 0.1285 | BioGRID | 3498641 |
Curated By
- BioGRID