BAIT

CDH2

CDHN, N-cadherin, Ncad
cadherin 2
GO Process (19)
GO Function (9)
GO Component (15)
Mus musculus
PREY

JUP

Ctnng, D930025P04Rik, PG, RP23-392I3.6
junction plakoglobin
GO Process (17)
GO Function (7)
GO Component (21)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Loss of cadherin-binding proteins ?-catenin and plakoglobin in the heart leads to gap junction remodeling and arrhythmogenesis.

Swope D, Cheng L, Gao E, Li J, Radice GL

Arrhythmic right ventricular cardiomyopathy (ARVC) is a hereditary heart muscle disease that causes sudden cardiac death (SCD) in young people. Almost half of ARVC patients have a mutation in genes encoding cell adhesion proteins of the desmosome, including plakoglobin (JUP). We previously reported that cardiac tissue-specific plakoglobin (PG) knockout (PG CKO) mice have no apparent conduction abnormality and survive longer ... [more]

Mol Cell Biol Mar. 01, 2012; 32(6);1056-67 [Pubmed: 22252313]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDH2 JUP
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.912BioGRID
2670401

Curated By

  • BioGRID