An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity labeling reveals dynamic changes in the SQSTM1 protein network.

Rondon Ortiz AN, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang Z, Dorrian L, Emili A, Wolozin B

Sequestosome1 (SQSTM1) is an autophagy receptor that mediates degradation of intracellular cargo, including protein aggregates, through multiple protein interactions. These interactions form the SQSTM1 protein network, and these interactions are mediated by SQSTM1 functional interaction domains, which include LIR, PB1, UBA and KIR. Technological advances in cell biology continue to expand our knowledge of the SQSTM1 protein network and of ... [more]

J Biol Chem Aug. 02, 2024; ();107621 [Pubmed: 39098523]

Throughput

High Throughput

Additional Notes

BioID
FLAG-tagged human full-length TurboID::SQSTM1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SQSTM1 PDLIM5	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	4.95	BioGRID	3007042

Curated By

BioGRID

Interaction Summary

SQSTM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH2 domain binding [IDA]identical protein binding [IPI]protein binding [IPI]protein kinase C binding [IPI]protein kinase binding [IDA]protein serine/threonine kinase activity [NAS]receptor tyrosine kinase binding [TAS]ubiquitin binding [IDA]

Gene Ontology Cellular Component

PDLIM5

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [ISS]actinin binding [ISS]protein kinase C binding [ISS]