BAIT

S

spike, SARS-CoV2 S, SARS-CoV2 spike, S protein, surface, SARS-CoV-2 spike, SARS-CoV-2 S, SPIKE_SARS2, PRO_0000449646, GU280_gp02
Spike glycoprotein; Surface glycoprotein
COVID-19 Coronavirus Project
Synthetic Protein Interaction Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

CMTR2

AFT, FTSJD1, HMTr2, MTr2
cap methyltransferase 2
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identifications of novel host cell factors that interact with the receptor-binding domain of the SARS-CoV-2 spike protein.

Tang X, Liu Y, Wang J, Long T, Yee Mok BW, Huang Y, Peng Z, Jia Q, Liu C, So PK, Pui-Kam Tse S, Hei Ng C, Liu S, Sun F, Tang S, Yao ZP, Chen H, Guo Y

SARS-CoV-2 entry into host cells is facilitated by the interaction between the receptor-binding domain of its spike protein (CoV2-RBD) and host cell receptor, ACE2, promoting viral membrane fusion. The virus also uses endocytic pathways for entry, but the mediating host factors remain largely unknown. It is also unknown whether mutations in the RBD of SARS-CoV-2 variants promote interactions with additional ... [more]

J Biol Chem Jun. 01, 2024; 300(6);107390 [Pubmed: 38777146]

Throughput

  • High Throughput

Additional Notes

  • RBD
  • hit proteins preferentially bound to the Delta variant of GST-CoV2-RBD rather than the parental GST-CoV2-RBD

Curated By

  • BioGRID