PREY

HSPD1

CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13
heat shock 60kDa protein 1 (chaperonin)
GO Process (24)
GO Function (11)
GO Component (16)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

EZH2 K63-polyubiquitination affecting migration in extranodal natural killer/T-cell lymphoma.

Li B, Zhou Q, Wan Q, Qiao X, Chen S, Zhou J, Wuxiao Z, Luo L, Ng SB, Li J, Chng WJ

Overexpressed EZH2 is oncogenically involved in the pathogenesis of different cancerous contexts including extranodal natural killer/T cell lymphoma (ENKTL). However, the underlying mechanisms of EZH2 upregulation have not been fully clarified and it is still difficult to target EZH2 in ENKTL.Current study identifies an E3 ligase TRIP12 that triggers K63-linked polyubiquitination of EZH2 in ENKTL and unexpectedly, stabilizes EZH2. As ... [more]

Clin Epigenetics Nov. 29, 2023; 15(1);187 [Pubmed: 38031139]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EZH2 HSPD1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID