An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway.

Prosdocimi E, Carpanese V, Todesca LM, Varanita T, Bachmann M, Festa M, Bonesso D, Perez-Verdaguer M, Carrer A, Velle A, Peruzzo R, Muccioli S, Doni D, Leanza L, Costantini P, Stein F, Rettel M, Felipe A, Edwards MJ, Gulbins E, Cendron L, Romualdi C, Checchetto V, Szabo I

Kv1.3 is a multifunctional potassium channel implicated in multiple pathologies, including cancer. However, how it is involved in disease progression is not fully clear. We interrogated the interactome of Kv1.3 in intact cells using BioID proximity labeling, revealing that Kv1.3 interacts with STAT3- and p53-linked pathways. To prove the relevance of Kv1.3 and of its interactome in the context of ... [more]

Sci Adv Sep. 06, 2024; 10(36);eadn9361 [Pubmed: 39231216]

Throughput

High Throughput

Additional Notes

BioID
Hit proteins have a ratio greater than 2 [log2 fold change (FC) greater than 1] and a false discovery rate (FDR) less than 0.05

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CTTN KCNA3	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Hajdu P (2015)	Low	-	BioGRID	1520814

Curated By

BioGRID

Interaction Summary

KCNA3

Gene Ontology Biological Process

Gene Ontology Molecular Function

delayed rectifier potassium channel activity [IBA]voltage-gated ion channel activity [TAS]

Gene Ontology Cellular Component

CTTN