BAIT

KCNA3

HGK5, HLK3, HPCN3, HUKIII, KV1.3, MK3, PCN3, RP11-284N8.3
potassium voltage-gated channel, shaker-related subfamily, member 3
UPS Project
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SEC24B

SEC24
SEC24 family member B
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway.

Prosdocimi E, Carpanese V, Todesca LM, Varanita T, Bachmann M, Festa M, Bonesso D, Perez-Verdaguer M, Carrer A, Velle A, Peruzzo R, Muccioli S, Doni D, Leanza L, Costantini P, Stein F, Rettel M, Felipe A, Edwards MJ, Gulbins E, Cendron L, Romualdi C, Checchetto V, Szabo I

Kv1.3 is a multifunctional potassium channel implicated in multiple pathologies, including cancer. However, how it is involved in disease progression is not fully clear. We interrogated the interactome of Kv1.3 in intact cells using BioID proximity labeling, revealing that Kv1.3 interacts with STAT3- and p53-linked pathways. To prove the relevance of Kv1.3 and of its interactome in the context of ... [more]

Sci Adv Sep. 06, 2024; 10(36);eadn9361 [Pubmed: 39231216]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Hit proteins have a ratio greater than 2 [log2 fold change (FC) greater than 1] and a false discovery rate (FDR) less than 0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SEC24B KCNA3
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID