BAIT

TIMP2

CSC-21K, DDC8, KIAA1731NL
TIMP metallopeptidase inhibitor 2
GO Process (3)
GO Function (1)
GO Component (2)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Mapping Extracellular Protein-Protein Interactions Using Extracellular Proximity Labeling (ePL).

Peeney D, Gurung S, Rich JA, Coates-Park S, Liu Y, Toor J, Jones J, Richie CT, Jenkins LM, Stetler-Stevenson WG

Proximity labeling (PL) has given researchers the tools to explore protein-protein interactions (PPIs) in living systems; however, most PL studies are performed on intracellular targets. We have adapted the original PL method to investigate PPIs within the extracellular compartment, which we term extracellular PL (ePL). To demonstrate the utility of this modified technique, we investigated the interactome of the matrisome ... [more]

J Proteome Res Sep. 05, 2024; (); [Pubmed: 39238192]

Throughput

  • High Throughput

Additional Notes

  • BioID Extracellular Proximity Labeling (ePL)
  • High confidence predominant proximal interactors for TIMP2 in HT1080 cells (Table S4).

Curated By

  • BioGRID