BAIT

NSP8

ORF1ab, ORF1ab-nsp8, SARS-CoV2 nsp8, SARS-CoV-2 nsp8, R1AB_SARS2, PRO_0000449626, GU280_gp01_nsp8
Non-structural protein 8
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

MRPS5

MRP-S5, S5mt
mitochondrial ribosomal protein S5
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

In Vitro Reconstitution and Analysis of SARS-CoV-2/Host Protein-Protein Interactions.

Moradi SV, Wu Y, Walden P, Cui Z, Johnston WA, Petrov D, Alexandrov K

The emergence of viral threats such as Ebola, ZIKA, and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) requires a rapid and efficient approach for elucidating mechanisms of pathogenesis and development of therapeutics. In this context, cell-free protein synthesis (CFPS) holds a promise to resolve the bottlenecks of multiplexed protein production and interaction analysis among host and pathogen proteins. Here, we applied ... [more]

ACS Omega Jul. 18, 2023; 8(28);25009-25019 [Pubmed: 37483225]

Throughput

  • Low Throughput

Additional Notes

  • AlphaLISA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP8 MRPS5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.6364BioGRID
2652547
NSP8 MRPS5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.6364BioGRID
2875545

Curated By

  • BioGRID