BAIT

M

mem, membrane, SARS-CoV2 M, M protein, SARS-CoV-2 M, VME1_SARS2, PRO_0000449652, GU280_gp05
Membrane glycoprotein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

SLC30A7

ZNT7, ZnT-7, ZnTL2, RP11-30G24.1
solute carrier family 30 (zinc transporter), member 7
GO Process (2)
GO Function (0)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

In Vitro Reconstitution and Analysis of SARS-CoV-2/Host Protein-Protein Interactions.

Moradi SV, Wu Y, Walden P, Cui Z, Johnston WA, Petrov D, Alexandrov K

The emergence of viral threats such as Ebola, ZIKA, and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) requires a rapid and efficient approach for elucidating mechanisms of pathogenesis and development of therapeutics. In this context, cell-free protein synthesis (CFPS) holds a promise to resolve the bottlenecks of multiplexed protein production and interaction analysis among host and pathogen proteins. Here, we applied ... [more]

ACS Omega Jul. 18, 2023; 8(28);25009-25019 [Pubmed: 37483225]

Throughput

  • Low Throughput

Additional Notes

  • AlphaLISA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
M SLC30A7
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.767BioGRID
2875319
M SLC30A7
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.767BioGRID
2652362
M SLC30A7
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3208715
M SLC30A7
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High11BioGRID
2808081

Curated By

  • BioGRID