Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Contribution of DEAF1 structural domains to the interaction with the breast cancer oncogene LMO4.

Cubeddu L, Joseph S, Richard DJ, Matthews JM

The proteins LMO4 and DEAF1 contribute to the proliferation of mammary epithelial cells. During breast cancer LMO4 is upregulated, affecting its interaction with other protein partners. This may set cells on a path to tumour formation. LMO4 and DEAF1 interact, but it is unknown how they cooperate to regulate cell proliferation. In this study, we identify a specific LMO4-binding domain ... [more]

PLoS One Jun. 23, 2012; 7(6);e39218 [Pubmed: 22723967]

Throughput

  • Low Throughput

Curated By

  • BioGRID