BAIT

ORF1AB

ORF1ab polyprotein, 1ab, SARS-CoV2 ORF1ab, SARS-CoV-2 ORF1ab, Replicase, R1AB_SARS2, PRO_0000449618, GU280_gp01
Replicase polyprotein 1ab
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

TIA1

TIA-1, WDM
TIA1 cytotoxic granule-associated RNA binding protein
Alzheimer's Disease Project
GO Process (1)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9.

Schmidt N, Ganskih S, Wei Y, Gabel A, Zielinski S, Keshishian H, Lareau CA, Zimmermann L, Makroczyova J, Pearce C, Krey K, Hennig T, Stegmaier S, Moyon L, Horlacher M, Werner S, Aydin J, Olguin-Nava M, Potabattula R, Kibe A, Doelken L, Smyth RP, Caliskan N, Marsico A, Krempl C, Bodem J, Pichlmair A, Carr SA, Chlanda P, Erhard F, Munschauer M

Regulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-2 RNAs. We find that the host protein SND1 binds the 5' end of negative-sense viral RNA and is required for SARS-CoV-2 RNA synthesis. SND1-depleted cells form smaller replication organelles ... [more]

Cell Oct. 26, 2023; 186(22);4834-4850.e23 [Pubmed: 37794589]

Throughput

  • High Throughput

Additional Notes

  • RNA capture (RAP)-MS was carried out to identify high confidence protein interactors of SARS-CoV-2 gRNA (pulled down with antisense probes to ORF1ab)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF1AB TIA1
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3834578
ORF1AB TIA1
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
-

Curated By

  • BioGRID