BAIT

HNRNPU

HNRPU, SAF-A, U21.1, hnRNP U, RP11-11N7.3
heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Homo sapiens
PREY

DMGDH

DMGDHD, ME2GLYDH
dimethylglycine dehydrogenase
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

DSBSO-Based XL-MS Analysis of Breast Cancer PDX Tissues to Delineate Protein Interaction Network in Clinical Samples.

Jiao F, Yu C, Wheat A, Chen L, Lih TM, Zhang H, Huang L

Protein-protein interactions (PPIs) are fundamental to understanding biological systems as protein complexes are the active molecular modules critical for carrying out cellular functions. Dysfunctional PPIs have been associated with various diseases including cancer. Systems-wide PPI analysis not only sheds light on pathological mechanisms, but also represents a paradigm in identifying potential therapeutic targets. In recent years, cross-linking mass spectrometry (XL-MS) ... [more]

J Proteome Res Aug. 02, 2024; 23(8);3269-3279 [Pubmed: 38334954]

Throughput

  • High Throughput

Additional Notes

  • Basal subtype
  • DSBSO-based XL-MS to identify protein interactions in breast cancer patient-derived xenograft (PDX) model.
  • High confidence protein interactions had an FDR of 1.8%.

Curated By

  • BioGRID