RABGEF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSP90AB1
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- axon guidance [TAS]
- innate immune response [TAS]
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- regulation of interferon-gamma-mediated signaling pathway [IMP]
- regulation of type I interferon-mediated signaling pathway [IMP]
- response to unfolded protein [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
DSBSO-Based XL-MS Analysis of Breast Cancer PDX Tissues to Delineate Protein Interaction Network in Clinical Samples.
Protein-protein interactions (PPIs) are fundamental to understanding biological systems as protein complexes are the active molecular modules critical for carrying out cellular functions. Dysfunctional PPIs have been associated with various diseases including cancer. Systems-wide PPI analysis not only sheds light on pathological mechanisms, but also represents a paradigm in identifying potential therapeutic targets. In recent years, cross-linking mass spectrometry (XL-MS) ... [more]
Throughput
- High Throughput
Additional Notes
- DSBSO-based XL-MS to identify protein interactions in breast cancer patient-derived xenograft (PDX) model.
- High confidence protein interactions had an FDR of 1.8%.
- Luminal subtype
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HSP90AB1 RABGEF1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -2.03 | BioGRID | 2538950 |
Curated By
- BioGRID