SFN
Gene Ontology Biological Process
- apoptotic process [TAS]
- establishment of skin barrier [ISS]
- intrinsic apoptotic signaling pathway [TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- membrane organization [TAS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of protein kinase activity [TAS]
- negative regulation of protein serine/threonine kinase activity [TAS]
- positive regulation of epidermal cell differentiation [ISS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of epidermal cell division [ISS]
- release of cytochrome c from mitochondria [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
APC
Gene Ontology Biological Process
- apoptotic process [TAS]
- canonical Wnt signaling pathway [IC, NAS]
- cell adhesion [NAS]
- cell cycle arrest [IDA]
- cell migration [IMP]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular response to DNA damage stimulus [IDA]
- mitotic cytokinesis [IMP]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of canonical Wnt signaling pathway [IGI]
- negative regulation of cell proliferation [IDA]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of microtubule depolymerization [IDA, IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of protein catabolic process [IC, IGI]
- positive regulation of pseudopodium assembly [IMP]
- protein complex assembly [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP, NAS]
- regulation of microtubule-based process [IMP]
- tight junction assembly [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
DSBSO-Based XL-MS Analysis of Breast Cancer PDX Tissues to Delineate Protein Interaction Network in Clinical Samples.
Protein-protein interactions (PPIs) are fundamental to understanding biological systems as protein complexes are the active molecular modules critical for carrying out cellular functions. Dysfunctional PPIs have been associated with various diseases including cancer. Systems-wide PPI analysis not only sheds light on pathological mechanisms, but also represents a paradigm in identifying potential therapeutic targets. In recent years, cross-linking mass spectrometry (XL-MS) ... [more]
Throughput
- High Throughput
Additional Notes
- DSBSO-based XL-MS to identify protein interactions in breast cancer patient-derived xenograft (PDX) model.
- High confidence protein interactions had an FDR of 1.8%.
- Luminal subtype
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SFN APC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 244895 | |
| SFN APC | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3534103 |
Curated By
- BioGRID