SIK2
Gene Ontology Biological Process
Gene Ontology Molecular Function
EIF4EBP1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- TOR signaling [IDA]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- insulin receptor signaling pathway [TAS]
- negative regulation of translational initiation [IDA]
- positive regulation of mitotic cell cycle [IMP]
- translation [TAS]
- translational initiation [TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Inhibiting eukaryotic initiation factor 5A (eIF5A) hypusination attenuated activation of the SIK2 (salt-inducible kinase 2)-p4E-BP1 pathway involved in ovarian cancer cell proliferation and migration.
Eukaryotic initiation factor 5A hypusine (eIF5AHyp) stimulates the translation of proline repeat motifs. Salt inducible kinase 2 (SIK2) containing a proline repeat motif is overexpressed in ovarian cancers, in which it promotes cell proliferation, migration, and invasion.Western blotting and dual luciferase analyses showed that depletion of eIF5AHyp by GC7 or eIF5A-targeting siRNA downregulated SIK2 level and decreased luciferase activity in ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID