Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Structural basis for the ubiquitination of G protein ?? subunits by KCTD5/Cullin3 E3 ligase.

Jiang W, Wang W, Kong Y, Zheng S

G protein-coupled receptor (GPCR) signaling is precisely controlled to avoid overstimulation that results in detrimental consequences. G?? signaling is negatively regulated by a Cullin3 (Cul3)-dependent E3 ligase, KCTD5, which triggers ubiquitination and degradation of free G??. Here, we report the cryo-electron microscopy structures of the KCTD5-G?? fusion complex and the KCTD7-Cul3 complex. KCTD5 in pentameric form engages symmetrically with five ... [more]

Sci Adv Jul. 14, 2023; 9(28);eadg8369 [Pubmed: 37450587]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GNG2 KCTD2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9979BioGRID
3052107

Curated By

  • BioGRID