BAIT

RNH203

Rnh2C, YLR154C
Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS3 that causes Aicardi-Goutieres syndrome
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SMC6

RHC18, DNA repair protein SMC6, L000004130, YLR383W
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

The Smc5/6 complex counteracts R-loop formation at highly transcribed genes in cooperation with RNase H2.

Roy S, Adhikary H, Isler S, D'Amours D

The R-loop is a common transcriptional by-product that consists of an RNA-DNA duplex joined to a displaced strand of genomic DNA. While the effects of R-loops on health and disease are well established, there is still an incomplete understanding of the cellular processes responsible for their removal from eukaryotic genomes. Here, we show that a core regulator of chromosome architecture ... [more]

Elife Oct. 15, 2024; 13(); [Pubmed: 39404251]

Throughput

  • Low Throughput

Ontology Terms

  • chromosome/plasmid maintenance (APO:0000143)
  • vegetative growth (APO:0000106)

Additional Notes

  • genetic complex
  • simultaneous loss of Smc5/6 complex and RNase H activities results in severe growth defects in yeast

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMC6 RNH203
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1239BioGRID
2439552

Curated By

  • BioGRID