Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction.

Yang W, Zhuang Y, Wu H, Su S, Li Y, Wang C, Tian Z, Peng L, Zhang X, Liu J, Pei X, Yuan W, Hu X, Meng B, Li D, Zhang Y, Shan H, Pan Z, Lu Y

Speckle-type pox virus and zinc finger (POZ) protein (SPOP), a substrate recognition adaptor of cullin-3 (CUL3)/RING-type E3 ligase complex, is investigated for its role in cardiac fibrosis in our study. Cardiac fibroblasts (CFs) activation was achieved with TGF-?1 (20 ng/mL) and MI mouse model was established by ligation of the left anterior descending coronary, and lentivirus was employed to mediate interference ... [more]

Cell Chem Biol Oct. 19, 2023; 30(10);1248-1260.e4 [Pubmed: 37442135]

Throughput

  • High Throughput

Curated By

  • BioGRID