BAIT
SPOP
AI315626, Pcif1, TEF2, RP23-67E18.3
speckle-type POZ protein
GO Process (7)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- glucose homeostasis [IGI]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [IGI]
- positive regulation of type B pancreatic cell apoptotic process [IGI]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IGI, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
JUN
AP-1, Junc, c-jun, RP23-135A21.5
jun proto-oncogene
GO Process (43)
GO Function (21)
GO Component (7)
Gene Ontology Biological Process
- SMAD protein import into nucleus [ISO]
- SMAD protein signal transduction [ISO]
- angiogenesis [IMP, ISO]
- axon regeneration [IMP]
- cellular process [IMP]
- cellular response to calcium ion [IDA]
- cellular response to potassium ion starvation [ISO]
- leading edge cell differentiation [IMP]
- learning [ISO]
- liver development [IMP]
- membrane depolarization [ISO]
- microglial cell activation [IMP]
- monocyte differentiation [IMP]
- negative regulation by host of viral transcription [ISO]
- negative regulation of DNA binding [ISO]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IGI]
- negative regulation of neuron apoptotic process [IMP]
- negative regulation of protein autophosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- outflow tract morphogenesis [IMP]
- positive regulation by host of viral transcription [ISO]
- positive regulation of DNA replication [ISO]
- positive regulation of Rho GTPase activity [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of endothelial cell proliferation [IMP]
- positive regulation of fibroblast proliferation [IDA, IGI]
- positive regulation of monocyte differentiation [ISO]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of cell cycle [IMP]
- regulation of transcription, DNA-templated [IMP]
- release of cytochrome c from mitochondria [ISO]
- response to drug [IDA]
- response to muscle stretch [IDA]
- response to radiation [ISO]
- transcription from RNA polymerase II promoter [ISO]
- transcription, DNA-templated [ISO]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- HMG box domain binding [ISO]
- R-SMAD binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- Rho GTPase activator activity [ISO]
- cAMP response element binding [ISO]
- chromatin binding [IDA]
- double-stranded DNA binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- DNA binding [IDA]
- HMG box domain binding [ISO]
- R-SMAD binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- Rho GTPase activator activity [ISO]
- cAMP response element binding [ISO]
- chromatin binding [IDA]
- double-stranded DNA binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [IDA, ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction.
Speckle-type pox virus and zinc finger (POZ) protein (SPOP), a substrate recognition adaptor of cullin-3 (CUL3)/RING-type E3 ligase complex, is investigated for its role in cardiac fibrosis in our study. Cardiac fibroblasts (CFs) activation was achieved with TGF-?1 (20 ng/mL) and MI mouse model was established by ligation of the left anterior descending coronary, and lentivirus was employed to mediate interference ... [more]
Cell Chem Biol Oct. 19, 2023; 30(10);1248-1260.e4 [Pubmed: 37442135]
Throughput
- High Throughput
Curated By
- BioGRID