BAIT

ERBB2

CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
erb-b2 receptor tyrosine kinase 2
GO Process (30)
GO Function (12)
GO Component (7)
Homo sapiens
PREY

MCAM

CD146, MUC18
melanoma cell adhesion molecule
GO Process (4)
GO Function (0)
GO Component (3)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Extracellular proximal interaction profiling by cell surface-targeted TurboID reveals LDLR as a partner of liganded EGFR.

Al Mismar R, Samavarchi-Tehrani P, Seale B, Kasmaeifar V, Martin CE, Gingras AC

Plasma membrane proteins play pivotal roles in receiving and transducing signals from other cells and from the environment and are vital for cellular functionality. Enzyme-based, proximity-dependent approaches, such as biotin identification (BioID), combined with mass spectrometry have begun to illuminate the landscape of proximal protein interactions within intracellular compartments. To extend the potential of these approaches to study the extracellular ... [more]

Sci Signal Nov. 05, 2024; 17(861);eadl6164 [Pubmed: 39499777]

Throughput

  • High Throughput

Additional Notes

  • Extracellular TurboID (ecTurboID)
  • High confidence interactions had a 1% BFDR and a minimum of two spectral counts.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MCAM ERBB2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3643194

Curated By

  • BioGRID