BAIT

ERBB2

CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
erb-b2 receptor tyrosine kinase 2
GO Process (30)
GO Function (12)
GO Component (7)
Homo sapiens
PREY

EPHA2

ARCC2, CTPA, CTPP1, CTRCT6, ECK
EPH receptor A2
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Extracellular proximal interaction profiling by cell surface-targeted TurboID reveals LDLR as a partner of liganded EGFR.

Al Mismar R, Samavarchi-Tehrani P, Seale B, Kasmaeifar V, Martin CE, Gingras AC

Plasma membrane proteins play pivotal roles in receiving and transducing signals from other cells and from the environment and are vital for cellular functionality. Enzyme-based, proximity-dependent approaches, such as biotin identification (BioID), combined with mass spectrometry have begun to illuminate the landscape of proximal protein interactions within intracellular compartments. To extend the potential of these approaches to study the extracellular ... [more]

Sci Signal Nov. 05, 2024; 17(861);eadl6164 [Pubmed: 39499777]

Throughput

  • High Throughput

Additional Notes

  • Extracellular TurboID (ecTurboID)
  • High confidence interactions had a 1% BFDR and a minimum of two spectral counts.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ERBB2 EPHA2
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

High-BioGRID
2641008
ERBB2 EPHA2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
2575037
EPHA2 ERBB2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3730908

Curated By

  • BioGRID