An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

RNF167-mediated ubiquitination of Tollip inhibits TNF-?-triggered NF-?B and MAPK activation.

Yan Z, Dai J, Wang J, Feng Q, Wang Y, Han T, Wu C

Toll-interacting protein (Tollip) is a multifunctional regulator in cellular activities. However, whether its functions are subjected to post-translational modifications remains elusive. Here, we identified ubiquitination as a post-translational modification on Tollip. We found that Tollip interacted with ring finger protein 167 (RNF167) through its C-terminal coupling of ubiquitin to ER degradation (CUE) domain, and RNF167 functioned as the potential E3 ... [more]

FASEB J Aug. 01, 2023; 37(8);e23089 [Pubmed: 37410058]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RNF149 TOLLIP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kim J (2023)	High	14.61	BioGRID	3629624

Curated By

BioGRID

Interaction Summary

TOLLIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

Toll-like receptor binding [IPI]kinase binding [IPI]protein binding [IPI]signal transducer activity [NAS]

Gene Ontology Cellular Component

RNF149