PREY

KIF21A

kinesin family member 21A
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Short-lived AUF1 p42-binding mRNAs of RANKL and BCL6 have two distinct instability elements each.

Dogar AM, Pauchard-Batschulat R, Grisoni-Neupert B, Richman L, Paillusson A, Pradervand S, Hagenbuechle O, Ambrosini G, Schmid CD, Bucher P, Kuehn LC

Regulation of mRNA stability by RNA-protein interactions contributes significantly to quantitative aspects of gene expression. We have identified potential mRNA targets of the AU-rich element binding protein AUF1. Myc-tagged AUF1 p42 was induced in mouse NIH/3T3 cells and RNA-protein complexes isolated using anti-myc tag antibody beads. Bound mRNAs were analyzed with Affymetrix microarrays. We have identified 508 potential target mRNAs ... [more]

PLoS ONE Nov. 13, 2018; 13(11);e0206823 [Pubmed: 30418981]

Throughput

  • High Throughput

Curated By

  • BioGRID