BAIT

HNRNPD

Auf1, Hnrpd
heterogeneous nuclear ribonucleoprotein D
Mus musculus
PREY

ZBTB44

6030404E16Rik, Btbd15, E230011F09
zinc finger and BTB domain containing 44
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Short-lived AUF1 p42-binding mRNAs of RANKL and BCL6 have two distinct instability elements each.

Dogar AM, Pauchard-Batschulat R, Grisoni-Neupert B, Richman L, Paillusson A, Pradervand S, Hagenbuechle O, Ambrosini G, Schmid CD, Bucher P, Kuehn LC

Regulation of mRNA stability by RNA-protein interactions contributes significantly to quantitative aspects of gene expression. We have identified potential mRNA targets of the AU-rich element binding protein AUF1. Myc-tagged AUF1 p42 was induced in mouse NIH/3T3 cells and RNA-protein complexes isolated using anti-myc tag antibody beads. Bound mRNAs were analyzed with Affymetrix microarrays. We have identified 508 potential target mRNAs ... [more]

PLoS ONE Nov. 13, 2018; 13(11);e0206823 [Pubmed: 30418981]

Throughput

  • High Throughput

Curated By

  • BioGRID