Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 viral genes Nsp6, Nsp8, and M compromise cellular ATP levels to impair survival and function of human pluripotent stem cell-derived cardiomyocytes.

Liu J, Wu S, Zhang Y, Wang C, Liu S, Wan J, Yang L

Cardiovascular complications significantly augment the overall COVID-19 mortality, largely due to the susceptibility of human cardiomyocytes (CMs) to SARS-CoV-2 virus. SARS-CoV-2 virus encodes 27 genes, whose specific impacts on CM health are not fully understood. This study elucidates the deleterious effects of SARS-CoV-2 genes Nsp6, M, and Nsp8 on human CMs.CMs were derived from human pluripotent stem cells (hPSCs), including ... [more]

Stem Cell Res Ther Sep. 13, 2023; 14(1);249 [Pubmed: 37705046]

Throughput

  • High Throughput

Additional Notes

  • Hit proteins were present in more than one replicate

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
M RPL30
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High67.33BioGRID
2791205

Curated By

  • BioGRID