NR6A1
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
DNMT3A
Gene Ontology Biological Process
- C-5 methylation of cytosine [IGI, ISO]
- DNA methylation [IDA, IGI, ISO]
- DNA methylation involved in embryo development [IMP]
- DNA methylation involved in gamete generation [IMP]
- DNA methylation on cytosine [ISO]
- DNA methylation on cytosine within a CG sequence [ISO]
- S-adenosylhomocysteine metabolic process [ISO]
- S-adenosylmethioninamine metabolic process [ISO]
- cellular response to amino acid stimulus [IDA]
- hypermethylation of CpG island [IDA]
- maintenance of DNA methylation [TAS]
- methylation [TAS]
- methylation-dependent chromatin silencing [IDA, IGI]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of cell death [ISO]
- regulation of gene expression by genetic imprinting [IDA, IMP]
- spermatogenesis [IMP]
Gene Ontology Molecular Function- DNA (cytosine-5-)-methyltransferase activity [IDA, IGI, ISO]
- DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates [ISO]
- DNA binding [IDA, ISO]
- chromatin binding [IDA, ISO]
- identical protein binding [ISO]
- methyltransferase activity [TAS]
- protein binding [IPI]
- unmethylated CpG binding [ISO]
- DNA (cytosine-5-)-methyltransferase activity [IDA, IGI, ISO]
- DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates [ISO]
- DNA binding [IDA, ISO]
- chromatin binding [IDA, ISO]
- identical protein binding [ISO]
- methyltransferase activity [TAS]
- protein binding [IPI]
- unmethylated CpG binding [ISO]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Differential recruitment of methyl CpG-binding domain factors and DNA methyltransferases by the orphan receptor germ cell nuclear factor initiates the repression and silencing of Oct4.
The pluripotency gene Oct4 encodes a key transcription factor that maintains self-renewal of embryonic stem cell (ESC) and is downregulated upon differentiation of ESCs and silenced in somatic cells. A combination of cis elements, transcription factors, and epigenetic modifications, such as DNA methylation, mediates Oct4 gene expression. Here, we show that the orphan nuclear receptor germ cell nuclear factor (GCNF) ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DNMT3A NR6A1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NR6A1 DNMT3A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NR6A1 DNMT3A | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID