BAIT
DNMT3A
MmuIIIA
DNA methyltransferase 3A
GO Process (17)
GO Function (8)
GO Component (9)
Gene Ontology Biological Process
- C-5 methylation of cytosine [IGI, ISO]
- DNA methylation [IDA, IGI, ISO]
- DNA methylation involved in embryo development [IMP]
- DNA methylation involved in gamete generation [IMP]
- DNA methylation on cytosine [ISO]
- DNA methylation on cytosine within a CG sequence [ISO]
- S-adenosylhomocysteine metabolic process [ISO]
- S-adenosylmethioninamine metabolic process [ISO]
- cellular response to amino acid stimulus [IDA]
- hypermethylation of CpG island [IDA]
- maintenance of DNA methylation [TAS]
- methylation [TAS]
- methylation-dependent chromatin silencing [IDA, IGI]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of cell death [ISO]
- regulation of gene expression by genetic imprinting [IDA, IMP]
- spermatogenesis [IMP]
Gene Ontology Molecular Function- DNA (cytosine-5-)-methyltransferase activity [IDA, IGI, ISO]
- DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates [ISO]
- DNA binding [IDA, ISO]
- chromatin binding [IDA, ISO]
- identical protein binding [ISO]
- methyltransferase activity [TAS]
- protein binding [IPI]
- unmethylated CpG binding [ISO]
- DNA (cytosine-5-)-methyltransferase activity [IDA, IGI, ISO]
- DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates [ISO]
- DNA binding [IDA, ISO]
- chromatin binding [IDA, ISO]
- identical protein binding [ISO]
- methyltransferase activity [TAS]
- protein binding [IPI]
- unmethylated CpG binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
POU5F1
NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
POU domain, class 5, transcription factor 1
GO Process (33)
GO Function (18)
GO Component (8)
Gene Ontology Biological Process
- BMP signaling pathway involved in heart induction [ISO]
- blastocyst growth [IGI]
- cardiac cell fate determination [ISO]
- cell fate commitment [IMP]
- cell fate commitment involved in formation of primary germ layer [ISO]
- ectodermal cell fate commitment [IDA]
- endodermal cell fate commitment [IDA]
- endodermal cell fate specification [ISO]
- germ-line stem cell maintenance [IMP]
- mRNA transcription from RNA polymerase II promoter [IDA]
- mesodermal cell fate commitment [IDA]
- negative regulation of calcium ion-dependent exocytosis [IDA]
- negative regulation of cell differentiation [IGI]
- negative regulation of gene silencing by miRNA [ISO]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of gene expression [ISO]
- regulation of heart induction by regulation of canonical Wnt signaling pathway [ISO]
- regulation of methylation-dependent chromatin silencing [ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA]
- stem cell maintenance [IMP]
- transcription from RNA polymerase II promoter [IDA, ISO]
- trophectodermal cell differentiation [IGI]
Gene Ontology Molecular Function- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
Mus musculus
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Differential recruitment of methyl CpG-binding domain factors and DNA methyltransferases by the orphan receptor germ cell nuclear factor initiates the repression and silencing of Oct4.
The pluripotency gene Oct4 encodes a key transcription factor that maintains self-renewal of embryonic stem cell (ESC) and is downregulated upon differentiation of ESCs and silenced in somatic cells. A combination of cis elements, transcription factors, and epigenetic modifications, such as DNA methylation, mediates Oct4 gene expression. Here, we show that the orphan nuclear receptor germ cell nuclear factor (GCNF) ... [more]
Stem Cells Jul. 01, 2011; 29(7);1041-51 [Pubmed: 21608077]
Throughput
- Low Throughput
Additional Notes
- ChIP
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POU5F1 DNMT3A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 471775 |
Curated By
- BioGRID