BAIT

YTHDF1

C20orf21, RP5-963E22.1
YTH N(6)-methyladenosine RNA binding protein 1
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

FXR2

FMR1L2, FXR2P
fragile X mental retardation, autosomal homolog 2
GO Process (1)
GO Function (5)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Spatiotemporal protein interactome profiling through condensation-enhanced photocrosslinking.

Li K, Xie X, Gao R, Chen Z, Yang M, Wen Z, Weng Y, Fan X, Zhang G, Liu L, Zeng X, Han Y, Cao M, Wang X, Li J, Yang Z, Li T, Chen PR

Resolving protein-protein interactions (PPIs) inside biomolecular condensates is crucial for elucidating their functions and regulation mechanisms. The transient nature of condensates and the multiple localizations of clients, however, have rendered it challenging to determine compartment-specific PPIs. Here we developed a condensation-enhanced, spatially directed, metabolic incorporation-assisted photocrosslinking strategy-termed DenseMAP-for spatiotemporally resolved dissection of the direct protein interactome within condensates. By leveraging ... [more]

Nat Chem Nov. 05, 2024; (); [Pubmed: 39501047]

Throughput

  • High Throughput

Additional Notes

  • XL-MS was carried out to identify high confidence protein interactors in the whole cell

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FXR2 YTHDF1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2814020
YTHDF1 FXR2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Low-BioGRID
2818993

Curated By

  • BioGRID