BAIT

YTHDF1

C20orf21, RP5-963E22.1
YTH N(6)-methyladenosine RNA binding protein 1
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

DHX30

DDX30, RETCOR
DEAH (Asp-Glu-Ala-His) box helicase 30
GO Process (2)
GO Function (5)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Spatiotemporal protein interactome profiling through condensation-enhanced photocrosslinking.

Li K, Xie X, Gao R, Chen Z, Yang M, Wen Z, Weng Y, Fan X, Zhang G, Liu L, Zeng X, Han Y, Cao M, Wang X, Li J, Yang Z, Li T, Chen PR

Resolving protein-protein interactions (PPIs) inside biomolecular condensates is crucial for elucidating their functions and regulation mechanisms. The transient nature of condensates and the multiple localizations of clients, however, have rendered it challenging to determine compartment-specific PPIs. Here we developed a condensation-enhanced, spatially directed, metabolic incorporation-assisted photocrosslinking strategy-termed DenseMAP-for spatiotemporally resolved dissection of the direct protein interactome within condensates. By leveraging ... [more]

Nat Chem Nov. 05, 2024; (); [Pubmed: 39501047]

Throughput

  • High Throughput

Additional Notes

  • XL-MS was carried out to identify high confidence protein interactors in the whole cell

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YTHDF1 DHX30
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
3204121

Curated By

  • BioGRID