Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

CircRNF10 triggers a positive feedback loop to facilitate progression of glioblastoma via redeploying the ferroptosis defense in GSCs.

Wang C, Zhang M, Liu Y, Cui D, Gao L, Jiang Y

Glioma exhibit heterogeneous susceptibility for targeted ferroptosis. How circRNAs alterations in glioma promote iron metabolism and ferroptosis defense remains unclarified.The highly enriched circRNAs in glioblastoma (GBM) were obtained through analysis of sequencing datasets. Quantitative real-time PCR (qRT-PCR) was used to determine the expression of circRNF10 in glioma and normal brain tissue. Both gain-of-function and loss-of-function studies were used to assess ... [more]

J Exp Clin Cancer Res Sep. 19, 2023; 42(1);242 [Pubmed: 37723588]

Throughput

  • Low Throughput

Additional Notes

  • Hit is circRNF10

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RNF10 ZBTB48
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Low/High-BioGRID
3765368

Curated By

  • BioGRID