An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

RIG-I promotes immune evasion of colon cancer by modulating PD-L1 ubiquitination.

Zhang Y, Zeng L, Wang M, Yang Z, Zhang H, Gao L, Zhang R, Liu J, Shan W, Chang Y, Liu L, Zhao Q, Li Y, Liu J

Colon cancer is one of the most prevalent cancers and exhibits high mortality worldwide. Despite the certain success in the immunotherapy of many tumor types, the limited response of colon cancer to immunotherapy remains a difficult problem. Retinoic acid-inducible gene-I (RIG-I) is a crucial component in innate antiviral immunity, but its role in antitumor immunity remains unclear. Here, in this ... [more]

J Immunother Cancer Sep. 01, 2023; 11(9); [Pubmed: 37758653]

Throughput

High Throughput

Additional Notes

Affinity capture MS was carried out to identify high confidence protein interactors for PD-L1 in the absence of RIG-I

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CD274 SSBP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Meng F (2020)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CD274

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SSBP1