An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

BioID-Screening Identifies PEAK1 and SHP2 as Components of the ALK Proximitome in Neuroblastoma Cells.

Uckun E, Siaw JT, Guan J, Anthonydhason V, Fuchs J, Wolfstetter G, Hallberg B, Palmer RH

Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase (RTK) that is mutated in approximately 10% of pediatric neuroblastoma (NB). To shed light on ALK-driven signaling processes, we employed BioID-based in vivo proximity labeling to identify molecules that interact intracellularly with ALK. NB-derived SK-N-AS and SK-N-BE(2) cells expressing inducible ALK-BirA* fusion proteins were generated and stimulated with ALKAL ligands in ... [more]

J Mol Biol Sep. 17, 2021; 433(19);167158 [Pubmed: 34273398]

Throughput

High Throughput

Additional Notes

BioID
SK-N-AS.ALK-BirA cells
SK-N-BE(2).ALK-BirA cells
Significant interactions (pval less than 0.05 and FC greater than 1.25 and PSMs greater than 2)
Significant interactions pval less than 0.05 and FC greater than 1.25 and PSMs greater than 2)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ALK VEZT	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Salokas K (2022)	High	0	BioGRID	3505079

Curated By

BioGRID

Interaction Summary

ALK

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB-inducing kinase activity [TAS]protein binding [IPI]protein tyrosine kinase activity [IDA]transmembrane receptor protein tyrosine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

VEZT