BAIT
ALK
CD246, NBLST3
anaplastic lymphoma receptor tyrosine kinase
GO Process (10)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- NIK/NF-kappaB signaling [TAS]
- activation of MAPK activity [TAS]
- cell proliferation [TAS]
- neuron development [TAS]
- peptidyl-tyrosine phosphorylation [IDA, TAS]
- phosphorylation [IDA, TAS]
- positive regulation of NF-kappaB transcription factor activity [TAS]
- protein autophosphorylation [IDA, TAS]
- regulation of apoptotic process [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PDE3B
HcGIP1, cGIPDE1
phosphodiesterase 3B, cGMP-inhibited
GO Process (9)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- blood coagulation [TAS]
- cAMP catabolic process [IEP]
- cellular response to insulin stimulus [ISS]
- insulin receptor signaling pathway [TAS]
- negative regulation of angiogenesis [IMP]
- negative regulation of cAMP-mediated signaling [IC]
- negative regulation of cell adhesion [IMP]
- negative regulation of cell adhesion mediated by integrin [IC]
- negative regulation of lipid catabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
BioID-Screening Identifies PEAK1 and SHP2 as Components of the ALK Proximitome in Neuroblastoma Cells.
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase (RTK) that is mutated in approximately 10% of pediatric neuroblastoma (NB). To shed light on ALK-driven signaling processes, we employed BioID-based in vivo proximity labeling to identify molecules that interact intracellularly with ALK. NB-derived SK-N-AS and SK-N-BE(2) cells expressing inducible ALK-BirA* fusion proteins were generated and stimulated with ALKAL ligands in ... [more]
J Mol Biol Sep. 17, 2021; 433(19);167158 [Pubmed: 34273398]
Throughput
- High Throughput
Additional Notes
- BioID
- SK-N-AS.ALK-BirA cells
- SK-N-BE(2).ALK-BirA cells
- Significant interactions (pval less than 0.05 and FC greater than 1.25 and PSMs greater than 2)
- Significant interactions pval less than 0.05 and FC greater than 1.25 and PSMs greater than 2)
Curated By
- BioGRID