Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The formin DAAM1 regulates the deubiquitinase activity of USP10 and integrin homeostasis.

Phillips AT, Boumil EF, Venkatesan A, Tilstra-Smith C, Castro N, Knox BE, Henty-Ridilla JL, Bernstein AM

The differentiation of fibroblasts into pathological myofibroblasts during wound healing is characterized by increased cell surface expression of ?v-integrins. Our previous studies found that the deubiquitinase (DUB) USP10 removes ubiquitin from ?v-integrins, leading to cell surface integrin accumulation, subsequent TGF?1 activation, and pathological myofibroblast differentiation. In this study, a yeast two-hybrid screen revealed a novel binding partner for USP10, the ... [more]

Eur J Cell Biol Dec. 01, 2023; 102(4);151347 [Pubmed: 37562219]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FSCN1 USP10
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
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Curated By

  • BioGRID