BAIT

RSA3

YLR221C
Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SNR30

L000001968
H/ACA box small nucleolar RNA (snoRNA); essential gene required for cleavage of 35S primary rRNA transcript to release the precursor to the 18S rRNA
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

The snoRNP chaperone snR190 and the Npa1 complex form a macromolecular assembly required for 60S ribosomal subunit maturation.

Hamze H, Jaafar M, Khreiss A, Dominique C, Bourdeaux J, Santo PE, Mendez-Godoy A, Kressler D, Humbert O, Plisson-Chastang C, Albert B, Henras AK, Henry Y

The early steps of large-ribosomal-subunit assembly feature among the least understood steps of ribosome synthesis in eukaryotes. In Saccharomyces cerevisiae, the box C/D chaperone small nucleolar ribonucleoprotein (snoRNP) snR190 and the Npa1 complex, composed of the ?-solenoid scaffold proteins Npa1 and Npa2, the DEAD-box helicase Dbp6, the RNA-binding protein Nop8, and Rsa3, are likely involved in early 25S ribosomal RNA ... [more]

Nucleic Acids Res Feb. 27, 2025; 53(5); [Pubmed: 40037705]

Throughput

  • Low Throughput

Curated By

  • BioGRID