Publication

Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.

Malaymar Pinar D, Goeoes H, Tan Z, Kumpula EP, Chowdhury I, Wang Z, Zhang Q, Salokas K, Keskitalo S, Wei GH, Kumbasar A, Varjosalo M

The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in a large number of interactions with other proteins and chromatin. However, despite their well-established significance, the NFIs' interactomes, their dynamics, and their functions have not been comprehensively examined. Here, we employed complementary omics-level techniques, ... [more]

Throughput

• High Throughput

Additional Notes

• BioID
• Filtered HCIs from 16h Biotinylated MAC3N tagged NFIA expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome
• Filtered HCIs from 3h Biotinylated MAC3N tagged NFIA expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome

Curated By

• BioGRID