BAIT
NFIB
CTF, HMGIC/NFIB, NF-I/B, NF1-B, NFI-B, NFI-RED, NFIB2, NFIB3, RP11-280O24.2
nuclear factor I/B
GO Process (15)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- Clara cell differentiation [ISS]
- Type I pneumocyte differentiation [ISS]
- Type II pneumocyte differentiation [ISS]
- anterior commissure morphogenesis [ISS]
- chondrocyte differentiation [ISS]
- commissural neuron axon guidance [ISS]
- glial cell differentiation [ISS]
- lung ciliated cell differentiation [ISS]
- negative regulation of DNA binding [IDA]
- negative regulation of epithelial cell proliferation involved in lung morphogenesis [ISS]
- negative regulation of mesenchymal cell proliferation involved in lung development [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- principal sensory nucleus of trigeminal nerve development [ISS]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription corepressor activity [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription corepressor activity [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
ZFHX4
ZFH4, ZHF4
zinc finger homeobox 4
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.
The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in a large number of interactions with other proteins and chromatin. However, despite their well-established significance, the NFIs' interactomes, their dynamics, and their functions have not been comprehensively examined. Here, we employed complementary omics-level techniques, ... [more]
Mol Cell Proteomics Jan. 01, 2025; 24(1);100890 [Pubmed: 39617063]
Throughput
- High Throughput
Additional Notes
- BioID
- Filtered HCIs from 16h Biotinylated MAC3N tagged NFIB expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome
- Filtered HCIs from 16h Biotinylated MAC3N tagged NFIB4 (a short isoform of NFIB) expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome
Curated By
- BioGRID