NFIC
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA]
RFX1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.
The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in a large number of interactions with other proteins and chromatin. However, despite their well-established significance, the NFIs' interactomes, their dynamics, and their functions have not been comprehensively examined. Here, we employed complementary omics-level techniques, ... [more]
Throughput
- High Throughput
Additional Notes
- BioID
- Filtered HCIs from 16h Biotinylated MAC3N tagged NFIC expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome
- Filtered HCIs from 3h Biotinylated MAC3N tagged NFIC expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RFX1 NFIC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID