BAIT
NFIC
CTF, CTF5, NF-I, NFI
nuclear factor I/C (CCAAT-binding transcription factor)
GO Process (3)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA]
Homo sapiens
PREY
CUX2
CDP2, CUTL2
cut-like homeobox 2
GO Process (8)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- cognition [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of dendrite morphogenesis [ISS]
- positive regulation of dendritic spine morphogenesis [ISS]
- positive regulation of excitatory postsynaptic membrane potential [ISS]
- positive regulation of gene expression [ISS]
- positive regulation of synapse assembly [ISS]
- short-term memory [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.
The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in a large number of interactions with other proteins and chromatin. However, despite their well-established significance, the NFIs' interactomes, their dynamics, and their functions have not been comprehensively examined. Here, we employed complementary omics-level techniques, ... [more]
Mol Cell Proteomics Jan. 01, 2025; 24(1);100890 [Pubmed: 39617063]
Throughput
- High Throughput
Additional Notes
- BioID
- Filtered HCIs from 16h Biotinylated MAC3N tagged NFIC expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.01 were discarded, but interactors that passed this filtering in any dataset were rescued. Interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in the experiments over three times higher than in CRAPome
Curated By
- BioGRID