NFIC
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [IDA]
ELAVL1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Nuclear Factor I Family Members are Key Transcription Factors Regulating Gene Expression.
The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in a large number of interactions with other proteins and chromatin. However, despite their well-established significance, the NFIs' interactomes, their dynamics, and their functions have not been comprehensively examined. Here, we employed complementary omics-level techniques, ... [more]
Throughput
- High Throughput
Additional Notes
- Filtered HCIs from Affinity purification experiment of MAC3N tagged NFIC expressing Flp-In T-REx 293 cell lines. Interactions with Saint assigned Bayesian FDR of over 0.05 were discarded and interactors that passed this filtering in any dataset were not rescued. Additionally, interactors with an average spectral count less than 3 were also discarded. Preys detected in 20 percent or more of CRAPome experiments were discarded unless they were rescued by having a spectral count in our experiments over three times higher than in CRAPome
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ELAVL1 NFIC | Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis. | High | - | BioGRID | 663469 |
Curated By
- BioGRID