BAIT

S

spike, SARS-CoV2 S, SARS-CoV2 spike, S protein, surface, SARS-CoV-2 spike, SARS-CoV-2 S, SPIKE_SARS2, PRO_0000449646, GU280_gp02
Spike glycoprotein; Surface glycoprotein
COVID-19 Coronavirus Project
Synthetic Protein Interaction Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

THOC6

BBIS, WDR58, fSAP35, PSEC0006
THO complex 6 homolog (Drosophila)
UPS Project
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Defining Distinct RNA-Protein Interactomes of SARS-CoV-2 Genomic and Subgenomic RNAs.

Whitworth IT, Knoener RA, Puray-Chavez M, Halfmann P, Romero S, Baddouh M, Scalf M, Kawaoka Y, Kutluay SB, Smith LM, Sherer NM

Host RNA binding proteins recognize viral RNA and play key roles in virus replication and antiviral mechanisms. SARS-CoV-2 generates a series of tiered subgenomic RNAs (sgRNAs), each encoding distinct viral protein(s) that regulate different aspects of viral replication. Here, for the first time, we demonstrate the successful isolation of SARS-CoV-2 genomic RNA and three distinct sgRNAs (N, S, and ORF8) ... [more]

J Proteome Res Jan. 05, 2024; 23(1);149-160 [Pubmed: 38043095]

Throughput

  • High Throughput

Additional Notes

  • 8 hrs PI
  • viral RNAs used to isolate host proteins which were then identified through mass spec

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
S THOC6
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

High-BioGRID
3558595

Curated By

  • BioGRID