BAIT

SNCA

NACP, PARK1, PARK4, PD1
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (45)
GO Function (20)
GO Component (16)

Gene Ontology Biological Process

Homo sapiens
PREY

CTNND1

CAS, CTNND, P120CAS, P120CTN, p120, p120(CAS), p120(CTN)
catenin (cadherin-associated protein), delta 1
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of ?-synuclein inclusions.

Lam I, Ndayisaba A, Lewis AJ, Fu Y, Sagredo GT, Kuzkina A, Zaccagnini L, Celikag M, Sandoe J, Sanz RL, Vahdatshoar A, Martin TD, Morshed N, Ichihashi T, Tripathi A, Ramalingam N, Oettgen-Suazo C, Bartels T, Boussouf M, Schaebinger M, Hallacli E, Jiang X, Verma A, Tea C, Wang Z, Hakozaki H, Yu X, Hyles K, Park C, Wang X, Theunissen TW, Wang H, Jaenisch R, Lindquist S, Stevens B, Stefanova N, Wenning G, van de Berg WDJ, Luk KC, Sanchez-Pernaute R, Gomez-Esteban JC, Felsky D, Kiyota Y, Sahni N, Yi SS, Chung CY, Stahlberg H, Ferrer I, Schoeneberg J, Elledge SJ, Dettmer U, Halliday GM, Bartels T, Khurana V

The heterogeneity of protein-rich inclusions and its significance in neurodegeneration is poorly understood. Standard patient-derived iPSC models develop inclusions neither reproducibly nor in a reasonable time frame. Here, we developed screenable iPSC "inclusionopathy" models utilizing piggyBac or targeted transgenes to rapidly induce CNS cells that express aggregation-prone proteins at brain-like levels. Inclusions and their effects on cell survival were trackable ... [more]

Neuron Sep. 04, 2024; 112(17);2886-2909.e16 [Pubmed: 39079530]

Quantitative Score

  • 2.0 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Scores from replicates: [MYTH run 1 repeat 1 ][ MYTH run 1 repeat 2 ][ MYTH run 1 repeat 3 ][ MYTH run 2 repeat 1 ][ MYTH run 2 repeat 2] = [NA][NA][NA][2][2]
  • Scores from technical replicates (2 rounds of MYTH, 3 technical replicates in first round, 2 technical replicates in second round)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SNCA CTNND1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID