BAIT
SNCA
NACP, PARK1, PARK4, PD1
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (45)
GO Function (20)
GO Component (16)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- calcium ion homeostasis [IDA]
- cellular response to copper ion [IDA]
- cellular response to epinephrine stimulus [TAS]
- cellular response to oxidative stress [IC]
- dopamine biosynthetic process [TAS]
- dopamine uptake involved in synaptic transmission [TAS]
- extracellular fibril organization [TAS]
- microglial cell activation [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of dopamine uptake involved in synaptic transmission [IDA]
- negative regulation of exocytosis [IMP]
- negative regulation of histone acetylation [IDA]
- negative regulation of microtubule polymerization [IDA]
- negative regulation of mitochondrial electron transport, NADH to ubiquinone [TAS]
- negative regulation of monooxygenase activity [IDA]
- negative regulation of norepinephrine uptake [IDA]
- negative regulation of platelet-derived growth factor receptor signaling pathway [IDA]
- negative regulation of serotonin uptake [IDA]
- negative regulation of thrombin receptor signaling pathway [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transporter activity [IDA]
- oxidation-reduction process [IDA]
- positive regulation of apoptotic process [TAS]
- positive regulation of endocytosis [IDA]
- positive regulation of glutathione peroxidase activity [IDA]
- positive regulation of hydrogen peroxide catabolic process [IDA]
- positive regulation of inositol phosphate biosynthetic process [IDA]
- positive regulation of peptidyl-serine phosphorylation [ISS]
- positive regulation of protein serine/threonine kinase activity [IDA]
- positive regulation of receptor recycling [IDA]
- positive regulation of release of sequestered calcium ion into cytosol [IDA]
- protein destabilization [IDA]
- receptor internalization [IDA]
- regulation of dopamine secretion [TAS]
- regulation of phospholipase activity [IDA]
- regulation of reactive oxygen species biosynthetic process [TAS]
- regulation of synaptic vesicle recycling [TAS]
- response to interferon-gamma [IDA]
- response to interleukin-1 [IDA]
- response to iron(II) ion [IDA]
- response to lipopolysaccharide [IDA]
- response to magnesium ion [IDA]
- synaptic vesicle endocytosis [ISS]
Gene Ontology Molecular Function- Hsp70 protein binding [IPI]
- alpha-tubulin binding [IPI]
- calcium ion binding [IDA]
- copper ion binding [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]
- dynein binding [IPI]
- fatty acid binding [IDA]
- ferrous iron binding [IDA]
- histone binding [IDA]
- identical protein binding [IPI]
- kinesin binding [IPI]
- magnesium ion binding [IDA]
- oxidoreductase activity [IDA]
- phospholipase D inhibitor activity [IDA]
- phospholipid binding [IDA]
- phosphoprotein binding [IDA]
- protein binding [IPI]
- tau protein binding [IDA]
- transcription regulatory region DNA binding [TAS]
- zinc ion binding [IDA]
- Hsp70 protein binding [IPI]
- alpha-tubulin binding [IPI]
- calcium ion binding [IDA]
- copper ion binding [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]
- dynein binding [IPI]
- fatty acid binding [IDA]
- ferrous iron binding [IDA]
- histone binding [IDA]
- identical protein binding [IPI]
- kinesin binding [IPI]
- magnesium ion binding [IDA]
- oxidoreductase activity [IDA]
- phospholipase D inhibitor activity [IDA]
- phospholipid binding [IDA]
- phosphoprotein binding [IDA]
- protein binding [IPI]
- tau protein binding [IDA]
- transcription regulatory region DNA binding [TAS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- axon [IDA]
- cell cortex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- extracellular region [TAS]
- fibril [IDA]
- growth cone [IDA]
- inclusion body [IDA]
- lysosome [TAS]
- mitochondrial respiratory chain complex I [TAS]
- mitochondrion [TAS]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- platelet alpha granule membrane [IDA]
Homo sapiens
PREY
RAB5A
RAB5
RAB5A, member RAS oncogene family
GO Process (13)
GO Function (4)
GO Component (16)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- Rab protein signal transduction [IBA]
- blood coagulation [TAS]
- early endosome to late endosome transport [IMP]
- endocytosis [IDA]
- intracellular protein transport [IBA]
- positive regulation of exocytosis [IMP]
- receptor internalization involved in canonical Wnt signaling pathway [IMP]
- regulation of endocytosis [IBA]
- regulation of endosome size [IMP]
- regulation of filopodium assembly [IDA]
- regulation of synaptic vesicle exocytosis [IMP]
- synaptic vesicle recycling [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axon [ISS]
- axon terminus [ISS]
- cytoplasm [IDA]
- cytoplasmic side of early endosome membrane [IMP]
- cytosol [IDA]
- dendrite [ISS]
- early endosome [IDA]
- endocytic vesicle [IBA]
- endosome [ISS]
- endosome membrane [TAS]
- extracellular vesicular exosome [IDA]
- neuronal cell body [ISS]
- plasma membrane [IBA]
- somatodendritic compartment [IDA]
- synaptic vesicle [ISS]
- terminal bouton [IDA]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of ?-synuclein inclusions.
The heterogeneity of protein-rich inclusions and its significance in neurodegeneration is poorly understood. Standard patient-derived iPSC models develop inclusions neither reproducibly nor in a reasonable time frame. Here, we developed screenable iPSC "inclusionopathy" models utilizing piggyBac or targeted transgenes to rapidly induce CNS cells that express aggregation-prone proteins at brain-like levels. Inclusions and their effects on cell survival were trackable ... [more]
Neuron Sep. 04, 2024; 112(17);2886-2909.e16 [Pubmed: 39079530]
Quantitative Score
- 2.0 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- Scores from replicates: [MYTH run 1 repeat 1 ][ MYTH run 1 repeat 2 ][ MYTH run 1 repeat 3 ][ MYTH run 2 repeat 1 ][ MYTH run 2 repeat 2] = [NA][NA][NA][2][2]
- Scores from technical replicates (2 rounds of MYTH, 3 technical replicates in first round, 2 technical replicates in second round)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAB5A SNCA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 732990 |
Curated By
- BioGRID