An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Defining Distinct RNA-Protein Interactomes of SARS-CoV-2 Genomic and Subgenomic RNAs.

Whitworth IT, Knoener RA, Puray-Chavez M, Halfmann P, Romero S, Baddouh M, Scalf M, Kawaoka Y, Kutluay SB, Smith LM, Sherer NM

Host RNA binding proteins recognize viral RNA and play key roles in virus replication and antiviral mechanisms. SARS-CoV-2 generates a series of tiered subgenomic RNAs (sgRNAs), each encoding distinct viral protein(s) that regulate different aspects of viral replication. Here, for the first time, we demonstrate the successful isolation of SARS-CoV-2 genomic RNA and three distinct sgRNAs (N, S, and ORF8) ... [more]

J Proteome Res Jan. 05, 2024; 23(1);149-160 [Pubmed: 38043095]

Throughput

High Throughput

Additional Notes

24 hrs PI
viral RNAs used to isolate proteins which were then identified through mass spec

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
N GAPVD1	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Whitworth IT (2023)	High	-	BioGRID	3559258

Curated By

BioGRID

Interaction Summary

N

GAPVD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activating protein binding [IMP]guanyl-nucleotide exchange factor activity [IMP]

Gene Ontology Cellular Component

Affinity Capture-RNA