An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer's disease.

Shafiq M, Zafar S, Younas N, Noor A, Puig B, Altmeppen HC, Schmitz M, Matschke J, Ferrer I, Glatzel M, Zerr I

High-density oligomers of the prion protein (HDPs) have previously been identified in brain tissues of patients with rapidly progressive Alzheimer's disease (rpAD). The current investigation aims at identifying interacting partners of HDPs in the rpAD brains to unravel the pathological involvement of HDPs in the rapid progression.HDPs from the frontal cortex tissues of rpAD brains were isolated using sucrose density ... [more]

Mol Neurodegener Feb. 22, 2021; 16(1);11 [Pubmed: 33618749]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRNP MAPRE2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ulbrich S (2018)	High	-	BioGRID	2842711

Curated By

BioGRID

Interaction Summary

PRNP

Gene Ontology Biological Process

Gene Ontology Molecular Function

copper ion binding [IDA, TAS]identical protein binding [IPI]microtubule binding [IDA]protein binding [IPI]tubulin binding [IDA]

Gene Ontology Cellular Component

MAPRE2