An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

LANCL1, a cell surface protein, promotes liver tumor initiation through FAM49B-Rac1 axis to suppress oxidative stress.

Huang H, Tsui YM, Ho DW, Chung CY, Sze KM, Lee E, Cheung GC, Zhang VX, Wang X, Lyu X, Ng IO

HCC is an aggressive cancer with a poor clinical outcome. Understanding the mechanisms that drive tumor initiation is important for improving treatment strategy. This study aimed to identify functional cell membrane proteins that promote HCC tumor initiation.Tailor-made siRNA library screening was performed for all membrane protein-encoding genes that are upregulated in human HCC (n = 134), with sphere formation as ... [more]

Hepatology Feb. 01, 2024; 79(2);323-340 [Pubmed: 37540188]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LANCL1 FAM49B	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huang H (2024)	High	-	BioGRID	-
FAM49B LANCL1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Huang H (2024)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

LANCL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled receptor activity [TAS]SH3 domain binding [IPI]glutathione binding [IDA]low-density lipoprotein particle receptor binding [IDA]protein binding [IPI]zinc ion binding [IDA]

Gene Ontology Cellular Component

FAM49B